Autoclave Essay Example | Topics and Well Written Essays - 500 words

Autoclave - Essay role modelThe relationship between temperature and pressure is a directly proportional one. In chemistry when the pressure of a gas rises, the temperature of the gas increase correspondingly too. With this principle in mind steam is a gas used in the autoclave chamber. Increasing its pressure in a closed system provide lead to increase in temperature. At higher temperatures water molecules of the steam become more invigorated. At this vigorous speed the molecules penetration escalates. This principle proves to be advantageous because it reduces the time interpreted by the things in the autoclave to sterilize (Hussain, 2009).As mentioned above dry waken and moist love piece of ass both provide sterilization but they differ in some manners. modify combust accounts on oxidizing molecule for its action. Dry heats mechanism of action is slower than moist heat. Dry heat penetrates walls of substances much slower than moist heat. Dry heat is used in sterilizing gla ss products, oils and powders. overdue to its slow mechanism of action sterilization by dry heat is done at 171 degrees centigrade for 1 hour and the time may increase depending on volume. Dry heat may also be used in the shape of an open flame used to sterilize inoculating loops, mouths of pipettes and culture tubes.On the other hand moist heat destroys organisms by the denaturing proteins and also by disrupting the membrane of lipids. Moist heat is favored because of its penetration powers. It is known that the most resistant spores require a temperature of 121C for around fractional an hour to denature. Hence moist heat application is more effective compared to dry heat sterilization. This can also be proven by the fact that moist heat, sterilization can be accomplished at lower temperatures in lesser time. Culture media, flammable and heat-sensitive items, liquids and dense loads can be sterilized by moist heat (Black,